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Thermocycling conditions for Sanger sequencing.
| Step | Temperature (°C) | Time | Cycle |
|---|---|---|---|
| 1 | 95 | 10 min | 1 |
| 2 | 94 | 30 sec | 3 |
| 64 | 30 sec | ||
| 72 | 45 sec | ||
| 3 | 94 | 30 sec | 5 |
| 62 | 30 sec | ||
| 72 | 45 sec | ||
| 4 | 94 | 30 sec | 10 |
| 60 | 30 sec | ||
| 72 | 45 sec | ||
| 5 | 94 | 30 sec | 17 |
| 58 | 30 sec | ||
| 72 | 45 sec | ||
| 6 | 72 | 5 min | 1 |
| 7 | 4 | Holds |
