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. 2019 Oct 25;20(6):5100–5110. doi: 10.3892/mmr.2019.10772

Figure 4.

Figure 4.

Intrathecal administration of JWH015 ameliorates impaired autophagy flux in bone cancer pain. (A) Representative western blotting images of LC3B and p62 in the spinal cord 12 h after treatment with JWH015. (B) Ratio of LC3B-II/LC3B-I and (C) quantification of p62 protein expression levels. #P<0.05 vs. Sham + vehicle group; *P<0.05 vs. Tumor + vehicle group. (D) Representative western blots of LC3B and p62 protein expression in the spinal cord at 0, 4, 8, 12, 24, 48 and 72 h after JWH015 (2 µg) injection. (E) Ratio of LC3B-II/LC3B-I and (F) quantification of p62. *P<0.05 vs. 0 h. (G) Representative blots of LC3B and p62 in the spinal cord with treatment of vehicle, Baf-A1 (10 nM), JWH015 (2 µg), and Baf-A1 (10 nM) + JWH015 (2 µg) in BCP mice on day 14. (H) Ratio of LC3B-II/LC3B-I. (I) Quantification of p62. *P<0.05 vs. tumor + vehicle group. For all western blots, β-actin was used as a loading control; data are expressed as the mean ± SD n=3 per group. LC3B, microtubule-associated protein 1 light chain 3β.