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. 2019 Oct 9;20(6):4855–4866. doi: 10.3892/mmr.2019.10734

Figure 2.

Figure 2.

Gefitinib treatment suppresses the proliferation, mobility and invasiveness of CSCC cells. (A) The proliferation ability of A431 cells treated with gefitinib (100 µM) was assessed by CCK-8 assays. (B) The representative images of crystal violet-stained cell colonies in A431 cells treated with gefitinib (10 or 20 µM) are presented (scale bar, 50 µm). (C) Colony formation ability of A431 cells following treatment with gefitinib (100 µM) or with DMSO as a control for different time-points. (D) The effect of gefitinib (50 or 100 µM) on A431 cell mobility for indicated time-points is presented (scale bar, 100 µm). (E) Invasive and migratory capacities of A431 cells treated with gefitinib (10, 20, or 50 µM) or with DMSO as a control were detected by Matrigel-coated or Matrigel-non-coated Transwell assays, respectively (scale bar, 50 µm). (F) qPCR analysis of the expression of N-cadherin and vimentin. ** P<0.01. CSCC, cutaneous squamous cell carcinoma.