Fig. 3.

MGMT expression correlates with PI3K-AKT pathway activation. a Total protein from parental GBM-PDX subcutaneous tumor lysates was used to assess the differential expression of MGMT and relative activation of p-AKT between the samples. b Densitometry was used to quantify the western blot results and all PDX tumors were normalized to GBM5. c Gene set variation analysis (GSVA) was used to determine gene set enrichment scores of the PI3K-AKT signaling pathway according to the methylation status of the MGMT promoter in clinical samples