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. 2019 Nov 14;16:42. doi: 10.1186/s12983-019-0341-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Hepatic fat deposition phenomenon in R. omeimontis tadpoles. a Typical morphology of a stage 31 R. omeimontis tadpole and its liver. b Micro-CT imaging of a stage 31 R. omeimontis tadpole. c Fat-specific stain by dithizone. Tissues with abundant neutral lipids are stained green. The liver is the only organ stained green. d-f Histological morphology of liver of R. omeimontis tadpoles: D, H&E staining; E, PAS staining; F, RO staining. g Distribution of triglycerides in R. omeimontis tadpoles (by TLC)