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. 2019 Nov 14;18:162. doi: 10.1186/s12943-019-1081-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — circLMTK2 promotes GC cell proliferation and tumourigenicity in vitro and in vivo. (a and b) Assessment of the proliferation of AGS and MGC-803 cells transfected with control or circLMTK2 siRNAs by CCK-8 assay. (c) Stable circLMTK2 overexpression promoted BGC-823 cell proliferation. OD, optical density. (d and e) Assessment of DNA synthesis using an EdU assay in MGC-803 and AGS cells transfected with control or circLMTK2 siRNAs. (f) Stable circLMTK2 overexpression promoted DNA synthesis in BGC-823 cells. Micrographs represent at least three experiments. Scale bar = 200 μm. (g and h) Colony formation assay using AGS and MGC-803 cells transfected with control or circLMTK2 siRNAs. (i) Stable circLMTK2 overexpression promoted BGC-823 cell colony formation. (j) Xenograft assay with BGC-823 stable cell lines. (k) circLMTK2 over-expression increased the volume of the xenograft tumours. (l) circLMTK2 over-expression increased the weight of the xenograft tumours. (a-l) Results are shown as the mean ± standard error of the mean (SEM) of three experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test)