Fig. 5.

circLMTK2 serves as a miRNA sponge for the miR-150-5p. (a) circRNA-miRNA-gene regulatory network from the CircNet database. (b) Left: circLMTK2 in GC cell lysate was pulled down and enriched with a circLMTK2- specific probe and then detected by qRT-PCR. Right: miR-150-5p was pulled down and enriched with a circLMTK2-specific probe and then detected by qRT-PCR. (c) A schematic drawing shows the putative binding sites of miR-150-5p with respect to circLMTK2. (d) A luciferase reporter assay was used to detect the luciferase activity of LUC-circRNA in HEK 293 T cells cotransfected with miRNA mimics. The data are the means ± SEM of three experiments. (e) The correlation between circLMTK2 and miR-150-5p levels in 120 GC patients. Pearson’s correlation coefficient values (r) and P values are as indicated