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. 2019 Nov 14;38:467. doi: 10.1186/s13046-019-1462-y

Table 2.

Primer sequences for RT-qPCR

Genes Primer sequences
NSD1 F: 5′-AGGTGTAGAACACGATCCCG-3′
R: 5′-AGCCGACCTTTAGATGCAGA-3’
Wnt10b F: 5′-CACTGGAGGTCCTGATCGATC-3’
R: 5′-CAGCCCCAAGGTAAGGCTGAC-3’
Wnt10b-promoter F: 5′-TTTTGGATCCCAAGGCCCTC-3’
R: 5′-GTTTGGCCCTAGCAGAGGTT-3’
GAPDH F: 5′-GAAGGTGAAGGTCGGAGTC-3’
R: 5′-GAAGATGGTGATGGGATTTC-3’

Note: F Forward, R Reverse, RT-qPCR Reverse transcription quantitative polymerase chain reaction, NSD1 Nuclear receptor binding SET domain protein 1, Wnt10b Wingless-related mouse mammary tumor virus integration site 10b, GAPDH Glyceraldehyde-3-phosphate dehydrogenase