Table 3.
Top 20 highest* and lowest* mean CLR OTU for the overall cohort and by age categories, and their linear correlation with age
| Rank Order* | OTU Label | Culture Status | Socransky Complex | Overall Cohort (N = 1219) | Age Categories (years) | Linear Correlation | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| 50–59 (N = 239) | 60–69 (N = 554) | ≥70 (N = 426) | p-value | Pearson r | p-value | |||||
|
CLR OTU Mean (SE) |
CLR OTU Mean (SE) |
CLR OTU Mean (SE) |
CLR OTU Mean (SE) |
|||||||
| 20 Most Abundant Species | ||||||||||
| 1 | Veillonella dispar | N | – | 8.25 (0.06) | 8.23 (0.13) | 8.11 (0.09) | 8.45 (0.10) | 0.045 | 0.08 | 0.008 |
| 2 | Streptococcus oralis | N | Y | 8.06 (0.05) | 8.40 (0.11) | 8.06 (0.08) | 7.87 (0.09) | 0.002 | −0.10 | <.001 |
| 3 | Veillonella parvula | N | P | 7.60 (0.07) | 7.48 (0.15) | 7.45 (0.09) | 7.86 (0.11) | 0.014 | 0.10 | 0.001 |
| 4 | Fusobacterium nucleatum_subsp._vincentii | N | O | 6.43 (0.08) | 6.21 (0.17) | 6.48 (0.11) | 6.50 (0.13) | 0.356 | 0.02 | 0.587 |
| 5 | Selenomonas sputigena | N | – | 5.63 (0.08) | 5.34 (0.17) | 5.44 (0.12) | 6.03 (0.14) | 0.001 | 0.10 | <.001 |
| 6 | Fusobacterium nucleatum_subsp._animalis | N | – | 5.39 (0.07) | 5.29 (0.14) | 5.29 (0.10) | 5.57 (0.12) | 0.136 | 0.10 | 0.106 |
| 7 | Campylobacter gracilis | N | O | 5.19 (0.05) | 5.02 (0.11) | 5.15 (0.07) | 5.33 (0.09) | 0.052 | 0.10 | 0.016 |
| 8 | Fusobacterium nucleatum_subsp._polymorphum | N | O | 5.19 (0.07) | 5.36 (0.15) | 5.28 (0.09) | 4.97 (0.12) | 0.052 | −0.10 | 0.019 |
| 9 | Prevotella oris | N | – | 5.08 (0.09) | 5.25 (0.18) | 5.05 (0.13) | 5.03 (0.16) | 0.646 | −0.10 | 0.118 |
| 10 | Streptococcus sanguinis | N | Y | 4.91 (0.07) | 5.57 (0.15) | 5.04 (0.10) | 4.38 (0.13) | <.001* | −0.18 | <.001* |
| 11 | Corynebacterium matruchotii | N | – | 4.81 (0.07) | 4.87 (0.17) | 4.86 (0.10) | 4.70 (0.12) | 0.550 | −0.04 | 0.171 |
| 12 | Selenomonas noxia | N | – | 4.81 (0.08) | 4.63 (0.18) | 4.69 (0.11) | 5.06 (0.13) | 0.049 | 0.10 | <.001 |
| 13 | Prevotella nigrescens | N | O | 4.31 (0.10) | 4.48 (0.21) | 4.28 (0.15) | 4.26 (0.18) | 0.714 | − 0.03 | 0.370 |
| 14 | Parvimonas micra | N | – | 4.29 (0.08) | 4.08 (0.17) | 4.35 (0.11) | 4.33 (0.12) | 0.401 | 0.00 | 0.967 |
| 15 | Rothia dentocariosa | U | – | 4.28 (0.09) | 4.46 (0.19) | 4.38 (0.13) | 4.04 (0.15) | 0.113 | −0.10 | 0.008 |
| 16 | Fusobacterium sp._oral_taxon_203 | N | – | 4.23 (0.10) | 4.08 (0.22) | 4.36 (0.15) | 4.16 (0.17) | 0.497 | −0.01 | 0.713 |
| 17 | Streptococcus gordonii | N | Y | 4.19 (0.08) | 3.93 (0.18) | 4.15 (0.12) | 4.40 (0.13) | 0.090 | 0.04 | 0.039 |
| 18 | Granulicatella adiacens | N | – | 4.15 (0.06) | 4.23 (0.14) | 4.11 (0.09) | 4.15 (0.10) | 0.749 | 0.00 | 0.991 |
| 19 | Streptococcus intermedius | N | Y | 3.94 (0.10) | 4.33 (0.21) | 3.86 (0.15) | 3.81 (0.16) | 0.119 | −0.10 | 0.016 |
| 20 | Actinomyces naeslundii | N | B | 3.85 (0.06) | 4.00 (0.14) | 3.88 (0.09) | 3.72 (0.11) | 0.230 | −0.10 | 0.008 |
| 20 Least Abundant Species | ||||||||||
| 1 | Porphyrobacter tepidarius | N | – | − 3.58 (0.03) | −3.51 (0.08) | − 3.65 (0.05) | − 3.54 (0.06) | 0.216 | 0.01 | 0.869 |
| 2 | Sphingomonas sp._oral_taxon_006 | P | – | − 3.55 (0.04) | − 3.49 (0.08) | − 3.60 (0.05) | − 3.52 (0.06) | 0.407 | −0.00 | 0.989 |
| 3 | Pyramidobacter piscolens | P | – | − 3.53 (0.04) | − 3.44 (0.10) | − 3.60 (0.06) | − 3.47 (0.07) | 0.251 | − 0.01 | 0.919 |
| 4 | Leptothrix sp._oral_taxon_025 | P | – | −3.50 (0.04) | − 3.37 (0.08) | − 3.53 (0.06) | −3.53 (0.06) | 0.216 | −0.04 | 0.221 |
| 5 | Treponema sp._oral_taxon_247 | P | – | −3.45 (0.05) | −3.35 (0.09) | − 3.50 (0.07) | −3.44 (0.08) | 0.436 | − 0.02 | 0.566 |
| 6 | Atopobium sp._oral_taxon_416 | P | – | −3.38 (0.05) | −3.45 (0.09) | − 3.44 (0.07) | −3.27 (0.09) | 0.202 | 0.10 | 0.042 |
| 7 | Brevundimonas diminuta | N | – | −3.30 (0.04) | −3.17 (0.09) | − 3.35 (0.06) | −3.30 (0.07) | 0.232 | −0.03 | 0.306 |
| 8 | Prevotella multiformis | N | – | −3.08 (0.06) | −3.12 (0.11) | − 3.10 (0.09) | −3.05 (0.10) | 0.901 | 0.03 | 0.359 |
| 9 | GN02_[G-2] sp._oral_taxon_873 | P | – | −3.06 (0.05) | −3.08 (0.12) | − 3.03 (0.08) | −3.10 (0.09) | 0.851 | −0.00 | 0.927 |
| 10 | Streptococcus sobrinus | U | – | −3.04 (0.06) | −3.21 (0.10) | − 3.19 (0.09) | −2.75 (0.14) | 0.005 | 0.11 | <.001* |
| 11 | Aggregatibacter actinomycetemcomitans | P | – | − 3.01 (0.07) | − 2.97 (0.15) | − 3.09 (0.10) | − 2.94 (0.11) | 0.575 | 0.03 | 0.349 |
| 12 | Fretibacterium sp._oral_taxon_361 | P | – | −2.98 (0.06) | −3.18 (0.10) | −2.97 (0.09) | − 2.88 (0.11) | 0.207 | 0.02 | 0.469 |
| 13 | Butyrivibrio sp._oral_taxon_080 | P | – | −2.96 (0.06) | −2.85 (0.13) | − 2.90 (0.09) | −3.10 (0.09) | 0.193 | −0.10 | 0.038 |
| 14 | Lactobacillus gasseri | N | – | −2.95 (0.06) | −3.08 (0.12) | −3.09 (0.09) | −2.70 (0.12) | 0.012 | 0.10 | 0.002 |
| 15 | Mitsuokella sp._oral_taxon_521 | N | – | −2.90 (0.06) | −2.91 (0.12) | − 2.92 (0.08) | −2.87 (0.11) | 0.929 | 0.02 | 0.603 |
| 16 | Microbacterium flavescens | P | – | −2.89 (0.04) | −2.73 (0.09) | − 2.89 (0.06) | −2.97 (0.07) | 0.151 | −0.10 | 0.051 |
| 17 | Prevotella sp._oral_taxon_475 | N | – | −2.87 (0.06) | −2.81 (0.12) | − 2.84 (0.09) | −2.95 (0.09) | 0.575 | −0.04 | 0.157 |
| 18 | Neisseria pharyngis | N | – | −2.87 (0.06) | −2.81 (0.13) | − 2.95 (0.09) | −2.80 (0.11) | 0.479 | 0.01 | 0.760 |
| 19 | Fretibacterium sp._oral_taxon_358 | U | – | −2.84 (0.07) | −2.87 (0.14) | − 2.88 (0.10) | −2.77 (0.12) | 0.708 | 0.02 | 0.494 |
| 20 | Treponema medium | P | – | −2.79 (0.06) | −2.85 (0.14) | − 2.74 (0.10) | −2.81 (0.10) | 0.777 | 0.02 | 0.579 |
*OTUs are ranked according to mean CLR OTU. The 20 most abundant OTUs have positive mean CRL; the 20 least abundant OTUs have negative mean CLR
The CLR OTU can be interpreted as a log [2] fold-difference for the given species relative to the overall compositional geometric mean. A mean CLR of 3 indicates a 8-fold [23] higher abundance, and a mean CLR of −3 indicates a 8-fold lower abundance, relative to the overall compositional geometric mean
SE standard error, Pearson r the Pearson product-moment correlation coefficient
p-values: bolded are significant at alpha .05; asterisk are significant at alpha 0.05 after Bonferroni correction
Culture status annotation in HOMD: N = named; U = unnamed; P = phylotyped
Socransky complex32: R = red; O = orange; P = purple; G = green; Y = yellow; B = blue; −-- = not part of the Socransky classification