Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 14;18:160. doi: 10.1186/s12943-019-1095-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — circGSK3β interacts with GSK3β and promotes metastasis by β-catenin. RNA pull-down experiment with ESCC cell lysate. The proteins were visualized by silver staining, and indicated spots were analyzed by mass spectrometry (a) or immunoblot of GSK3β (b). c QPCR detection of circGSK3β retrieved by GSK3β-specific antibody compared with immunoglobulin G (IgG) in the RIP assay. d RNA FISH assay of circGSK3β combined with immunofluorescence detection of GSK3β and β-Catenin in ESCC cells. e Graphical representation of three-dimensional structures of the interaction model of circGSK3β with GSK3β by SPOT-RNA. f β-catenin protein levels in ESCC cells with circGSK3β depletion or overexpression were detected by immunoblotting. g Immunoblot detection of indicated proteins in GSK3β-immunoprecipitated complex from lysates of ESCC cells with circGSK3β knockdown or overexpression. h and i ESCC cells with depletion of circGSK3β (Η) were transfected with β-catenin and circGSK3β-overexpressed cells (i) were transfected with control siRNA or siRNA against β-catenin. Migration and invasion abilities of ESCC cells were detected by Transwell and Matrigel invasion assay, respectively. j Statistical analyses of the association between circGSK3β and β-catenin expression in ESCC tissues. r, Pearson correlation coefficient; *P < 0.05, **P < 0.01, ***P < 0.001