Fig. 4.

Ablation of circGSK3β inhibits the tumorigenicity of ESCC cells. a and b ESCC cells with or without depletion of circGSK3β were grafted to the flank of nude mice by injecting the cells subcutaneously to observe tumor development, and volumes of tumor were determined and plotted as mean ± S.D. of six independent experiments. c The mRNA levels of circGSK3β in transplanted tumor tissues were determined by qRT-PCR. d Expression levels of β-catenin, E-cadherin and N-cadherin in tumors were determined by immunohistochemistry analysis. e Statistical analyses of the association between circGSK3β and β-catenin, E-cadherin or N-cadherin in transplanted tumor tissues. r, Pearson correlation coefficient. *P < 0.05. **P < 0.01, ***P < 0.001