Table 2.

Results from ANOVAs and MANOVAs comparing the anthocyanin and flavone contents among phenotypes in Barra (fully pigmented, PAL and WAL) and Breña (fully pigmented and WAL). Anthocyanin and flavone concentrations were obtained from HPLC analyses performed in four plants of each phenotype. Comparisons were made independently for each plant tissue. Total anthocyanins were considered for anthocyanin analyses, whereas main groups according to the C-glycoside flavone core were considered for flavone analyses (see Table 1)

	Tissue	Anthocyanins				Flavones
		ANOVA test				MANOVA test
		SS	d.f.	F	P	Wilk’s lambda	F	d.f.	P
Barra	Petals	6.403	2, 12	19.32	0.001a	0.215	1.735	4, 12	0.188
	Calyces	0.272	2, 12	8.249	0.009b	0.563	0.500	4, 12	0.834
	Leaves	0.001	2, 12	2.627	0.126	0.256	1.463	4, 12	0.266
	Stems	0.173	2, 12	17.28	< 0.001b	0.248	1.510	4, 12	0.251
Breña	Petals	15.11	1, 8	49.89	< 0.001	0.659	0.389	4, 8	0.808
	Calyces	0.106	1, 8	30.76	< 0.001	0.260	2.131	4, 8	0.280
	Leaves	0.004	1, 8	1.121	0.330	0.445	0.935	4, 8	0.544
	Stems	0.170	1, 8	10.71	0.017	0.121	5.448	4, 8	0.098

^apost hoc Tukey test showed significant differences between fully pigmented vs. PAL and WAL phenotypes (P < 0.05)

^bpost hoc Tukey test showed significant differences between WAL vs. fully pigmented and PAL phenotypes (P < 0.05)