Temporal evolution of
a simulation of cinnamycin bound to one POPE
molecule embedded in a POPC bilayer (simulations named PE_HyAsp).
The plot shows the change in the orientation of the HyAsp15 side chain,
Phe7(O), and Phe7(NH) over time (gray when the given group points
into the binding pocket and otherwise white), the distance between
the ammonium group of the lipid and the binding pocket (red line),
and the number of hydrogen bonds between the ammonium group of the
lipid and cinnamycin (blue line). The left y axis
describes the gray and white coloring, while the right y axis describes the red and blue curves. Above the plot, snapshots
of the complex are shown. It is interesting to notice how the HyAsp15
rotated in the 85 ns snapshot was followed by the Phe(NH) rotating
out of the binding pocket, as shown in the snapshot at 120 ns. The
lipid ammonium group has moved out of the ammonium-binding pocket
at 60 ns and has rebound again in the snapshot at 160 ns.