Figure 7.

BraR electrophoretic mobility shift assay (EMSA). (a) The nucleotide sequence of the vraDE promoter region and the DNA fragments used in this study. DNA fragments with or without the BraR‐binding site were used. Gray shadow, palindromic sequence: squares, −35, −10 box; *, vraD transcriptional start site; bold, vraD translation initiation codon. (b) EMSA of BraR using two DNA fragments. Fragments were labeled with DIG and incubated with recombinant unphosphorylated (rBraR, left) or phosphorylated BraR protein (rBraR‐phos, right) as described in the Section 2. After electrophoresis, DNA bands were detected as described in the Section 2