Table 1.
Strains used in this study
| Strains | Genotype | Reference |
|---|---|---|
| MW2 | Staphylococcus aureus clinical strain, methicillin‐resistant (mecA+) | Grundmann et al. (2006) |
| MM2070 | braRS inactivation in MW2, TCr | Kawada‐Matsuo, Yoshida, et al. (2013) |
| MM2156 | braS inactivation in MW2, TCr | Yoshida et al. (2011) |
| MM2005 | vraDE inactivation in MW2, TCr | Yoshida et al. (2011) |
| MM2139 | braRS (MW2) complementation in MM2070, TCr, CPr | This study |
| MM2141 | braRS (SAN8) complementation in MM2070, TCr, CPr | This study |
| MM2194 | braR (MW2) complementation in MM2070, TCr, CPr | This study |
| MM2195 | braR (SAN8) complementation in MM2070, TCr, CPr | This study |
| SAN1 | nisin A‐resistant mutant from MW2 | This study |
| MM2117 | braRS inactivation in SAN1, TCr | This study |
| MM2255 | braXRS (SAN1) complementation in MM2117, TCr, CPr | This study |
| SAN8 | nisin A‐resistant mutant from MW2 | This study |
| MM2120 | braRS inactivation in SAN8, TCr | This study |
| MM2145 | braRS (MW2) complementation in MM2120, TCr, CPr | This study |
| MM2147 | braRS (SAN8) complementation in MM2120, TCr, CPr | This study |
| MM2196 | braR (MW2) complementation in MM2120, TCr, CPr | This study |
| MM2197 | braR (SAN8) complementation in MM2120, TCr, CPr | This study |
| MM2116 | braS inactivation in SAN8, TCr | This study |
| SAN87 | nisin A‐resistant mutant from MW2 | This study |
| MM2256 | braRS inactivation in SAN87, TCr | This study |
| MM2258 | braRS (SAN87) complementation in MM2120, TCr, CPr | This study |
| MM2228 | lacZ gene fused with the promoter(MW2) of braRS in MW2, CPr | This study |
| MM2229 | lacZ gene fused with the promoter(SAN1) of braRS in MW2, CPr | This study |
| MM2264 | lacZ gene fused with the promoter(MW2) of braRS in MM2156, TCr, CPr | This study |
| MM2265 | lacZ gene fused with the promoter(MW2) of braRS in MM2116, TCr, CPr | This study |
| MM2199 | lacZ gene fused with the promoter(MW2) of vraDE in MW2, CPr | This study |
| MM2200 | lacZ gene fused with the promoter(MW2) of vraDE in SAN1, CPr | This study |
| MM2201 | lacZ gene fused with the promoter(MW2) of vraDE in SAN8, CPr | This study |
| MM2242 | lacZ gene fused with the promoter(MW2) of vraDE in SAN87, CPr | This study |
| MM2262 | lacZ gene fused with the promoter(MW2‐binding region less) of vraDE in MW2, CPr | This study |
| MM2263 | lacZ gene fused with the promoter(MW2‐binding region less) of vraDE in SAN8, CPr | This study |
| MM2266 | lacZ gene fused with the promoter(MW2‐binding region less) of vraDE in SAN87, CPr | This study |
| RN4220 | Restriction‐deficient transformation recipient | Kreiswirth et al. (1983) |
| MM2186 | braR (MW2) in RN4220, CPr | This study |
| MM2187 | braR (SAN8) in RN4220, CPr | This study |
| XL‐II | endA1 supE44 thi‐1 hsdR17 recA1 gyrA96 relA1 lac [F´ proAB lacIqZΔM15 Tn10 (TCr) Amy CPr] | Stratagene |
| MM1113 | His‐tag fused braD (MW2) gene in XL‐II, Ampr | This study |
| MM1114 | His‐tag fused braD (SAN8) gene in XL‐II, Ampr | This study |
Amp: ampicillin; CP: chloramphenicol; TC: tetracycline.