Figure 3.
Evaluation of ECM on the BADSC proliferation and differentiation. (b) The schematic summary of evaluating the influence of ECM hydrogel on BADSC behaviors. (c) Live and dead cell number counting after 1 day and 3 days of culture. (d) Alamar Blue assays demonstrated that both ECM and Col I could promote the proliferation of BADSCs. (e) Morphological observation of BADSCs with time grown on Col I or ECM hydrogel, as well as BrdU labeling of BADSCs at 3 days after seeding. (f) Immunofluorescent staining of cTnT (green) and CX43 (red) expression in BADSCs. ∗p < 0.05 and #p < 0.01 for “ECM vs. Col” at the same time points.