Figure 3.

Protein carbonylation in the mouse ovaries evidenced by fluorescence immunohistochemistry, using a specific antibody for DNP. (a) Representative images of young, reproductively aged and apocynin-treated aged mice. Note the substantial carbonyl labeling increase in ovarian stroma cells (white arrows) and antioxidant-mediated amelioration (arrowheads). (b) Average quantification of stained area (n = 4‐5 per group). Y: young; AG: aged; AA: aged apocynin. Data are presented as mean ± SEM. ^∗P < 0.05, compared with young female mice; ^#P < 0.05, compared with reproductively aged female mice.