Figure 4.

Fibrosis in mouse ovarian midsections stained by picrosirius red. (a) Representative images of young, reproductively aged and apocynin-treated aged mice. Note increased deposition of collagen fibers in the stroma of reproductively aged mice (arrows) and antioxidant-mediated amelioration (arrowheads). (b) Relative quantification of stained area assuming young mice equals 1 (n = 4 per group). Y: young; AG: aged; AA: aged apocynin. Bar = 100 μm. Data are presented as mean ± SEM. ^∗P < 0.05, compared with young female mice; ^#P < 0.05, compared with reproductively aged female mice.