Table 1.
List of primers used in RT-PCR reactions.
| Primers | Sequence | Annealing temperature | Fragment (bp) | |
|---|---|---|---|---|
| Col1α1 | Fwd | 5′-GACGCATGGCCAAGAAGACA-3′ | 60°C | 85 |
| Rev | 5′-CTCGGGTTTCCACGTCTCAC-3′ | |||
|
| ||||
| Col3α1 | Fwd | 5′-AGCTTTGTGCAAAGTGGAACC-3′ | 58°C | 114 |
| Rev | 5′-ATAGGACTGACCAAGGTGGC-3′ | |||
|
| ||||
| Col5α1 | Fwd | 5′-CCTGGTTCAGTGAATTCAAGCG-3′ | 60°C | 81 |
| Rev | 5′-TCATTTGTACCACGCCCACG-3′ | |||
|
| ||||
| TGF-β1 | Fwd | 5′-ATTCCTGGCGTTACCTTGG-3′ | 60°C | 120 |
| Rev | 5′-AGCCCTGTATTCCGTCTCCT-3′ | |||
|
| ||||
| TNF-α | Fwd | 5′-CCCTCACACTCAGATCATCTTCT-3′ | 60°C | 61 |
| Rev | 5′-GCTACGACGTGGGCTACAG-3′ | |||
|
| ||||
| IL-1β | Fwd | 5′-TGACGGACCCCAAAAGATGA-3′ | 60°C | 87 |
| Rev | 5′-TGCTGCGAGATTTGAAGCTG-3′ | |||
|
| ||||
| CCL5 | Fwd | 5′-TGCCCACGTCAAGGAGTATT-3′ | 60°C | 84 |
| Rev | 5′-ACTTGGCGGTTCCTTCGAG-3′ | |||
|
| ||||
| MMP2 | Fwd | 5′-TGTCGCCCCTAAAACAGACA-3′ | 58°C | 65 |
| Rev | 5′-TGGGGCAGCCATAGAAAGTG-3′ | |||
|
| ||||
| MMP9 | Fwd | 5′-CCTGGAACTCACACGACATCT-3′ | 62°C | 72 |
| Rev | 5′-CACGCCAGAAGAATTTGCCAT-3′ | |||
|
| ||||
| MMP12 | Fwd | 5′-GGGCTGCTCCCATGAATGAC-3′ | 56°C | 85 |
| Rev | 5′-GTCATTGGAATTCTGTCCTTTCCA-3′ | |||
|
| ||||
| TIMP1 | Fwd | 5′-GGTGTGCACAGTGTTTCCCTGTTT-3′ | 60°C | 72 |
| Rev | 5′-TCCGTCCACAAACAGTGAGTGTCA-3′ | |||
|
| ||||
| TIMP2 | Fwd | 5′-GGATTCAGTATGAGATCAAGC-3′ | 55°C | 145 |
| Rev | 5′-GCCTTTCCTGCAATTAGATAC-3′ | |||
|
| ||||
| 18S | Fwd | 5′-CGCCGCTAGAGGTGAAATTC-3′ | 60°C | 67 |
| Rev | 5′-CATTCTTGGCAAATGCTTTCG-3′ | |||
Fwd: forward; Rev: reverse; bp: base pairs.