Skip to main content
. 2019 Oct 24;2019:4745067. doi: 10.1155/2019/4745067

Figure 2.

Figure 2

Mitochondrial quality control in hepatocytes following LPS stimulation is dependent on iNOS induction. Autophagy marker LC3 (red) is induced in hepatocytes in an iNOS-dependent fashion following LPS exposure in vivo (12 h exposure, (a)) and in vitro (12 h exposure, (b)). Inhibition of iNOS in hepatocytes with the chemical inhibitor 1400 W (10 nM, 12 h cotreatment with LPS) suppresses induction of mitochondrial biogenesis-associated genes Pgc-1α, Nrf1, and Tfam following LPS expression as measured by q-RT-PCR (12 h LPS exposure, (c)). PGC-1α is induced in cultured hepatocytes following LPS exposure in an iNOS-dependent fashion (red staining, 12 h LPS exposure, (d)). ∗p < 0.05 compared to untreated controls; #p < 0.05 compared to LPS-treated alone. In immunofluorescent imaging, green staining is from cytoskeletal actin as measured by 488-conjugated phalloidin antibody.