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Effects of SAHA on T cells cultured in vitro under Th17 cell differentiation conditions. (a) Total splenocytes were extracted from the spleens of C57BL/6 mice and cultured with SAHA under Th17-polarizing conditions for 3 days. The cells were subjected to flow cytometric analysis of Th17 or Treg cells. Representative results are shown. (b) Three days after inducing Th17 cells, the protein levels of IL-17 and IL-10 were measured. ∗p < 0.05 and ∗∗∗p < 0.001.
