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. 2019 Oct 22;2019:8964015. doi: 10.1155/2019/8964015

Figure 5.

Figure 5

CKS2 promoted malignant behaviors of HCC cells. (a) The CKS2 expression of HCC cell lines was detected by western blotting. (b) The intensity of each bar in (a). (c) The CKS2 mRNA expression in HCC cell lines was detected by RT-qPCR. (d) CKS2 expression of MHCC97H cells transfected with KD-CKS2 plasmid was measured by western blotting. (e) The intensity of each bar in (d). (f) CKS2 expression of MHCC97H cells was detected by RT-qPCR. (g) CCK8 assay was conducted to determine the proliferation of MHCC97H cells transfected with NC and KD-CKS2. (h) The representative images of MHCC97H cell-derived colonies. (i) The colony number derived from cells transfected with NC or KD-CKS2. (j) The viability of MHCC97H cells with sorafenib treatment. (k) The viability of MHCC97H cells with regorafenib treatment. (l) The representative images of migration cells in the Transwell assay. (m) The number of migration cells. (n) The representative images of invasive cells in the Transwell assay. (o) The number of invasive cells. CKS2: cyclin-dependent kinase regulatory subunit 2. ∗P < 0.05; ∗∗P < 0.01.