Figure 4.

Blockade of TIM-1 on allogeneic donor T and iNKT cells ameliorates GVHD. (A-B) Overall survival (A) and GVHD score (B) of WT BALB/c recipient mice after transplantation with B6 (WT) Tcon cells and WT BM (▼), TIM-1^−/− Tcon cells and WT BM (●), or WT Tcon cells and TIM-1^−/− BM (▪). For the survival curve and GVHD, pooled data from 2 independent experiments are shown (TCD BM, n = 8 mice per group; WT Tcon cells + WT BM, n = 8 mice/group; TIM-1^−/− Tcon cells and WT BM, n = 7 mice per group; and WT Tcon cells and TIM-1^−/− BM, n = 8 mice per group). (C) Schematic of the experimental design of BALB/c recipient mice that received B6 or TIM-1^−/− iNKT cells in addition to B6 Tcon cells or TIM-1^−/− Tcon cells. (D-E) Overall survival (D) and GVHD score (E) of BALB/c recipient mice after injection of B6 Tcon cells alone (▪), B6 WT Tcon and B6 WT iNKT cells (25 000 enriched iNKT cells per mouse) (▼), B6 TIM-1^−/− Tcon and B6 WT iNKT cells (◯), or B6 WT Tcon and B6 TIM-1^−/− iNKT cells (●). GVHD scores for control mice that received only TCD BM are shown (☐). Statistical analysis for GVHD score is shown between the B6 WT Tcon cell plus B6 WT iNKT cell and B6 WT Tcon cell plus B6 TIM-1^−/− iNKT cell groups. Data are pooled from 2 independent experiments (n = 16 mice per group). Statistical evaluations of mouse survival (the log-rank test was used for Kaplan-Meier survival) and GVHD score (2-tailed Student t test) were performed. *P ≤ .05, ***P ≤ .01. Error bars indicate SEM.