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. 2019 Nov 7;10:1312. doi: 10.3389/fphar.2019.01312

Figure 4.

Figure 4

Iron addition (Fe-NTA) and iron chelation (DFP) altered the long-term potentiation (LTP) of prenatal ethanol exposure (PAE) rats compared to controls rats. (A) Effect of Fe-NTA on CA1 LTP of PAE and control rats. For long-term potentiation (LTP) analysis, slices from control or PAE rats were preincubated with 20 μM Fe-NTA, or saline (vehicle), during 40 min and continually perfused during the experiment. LTP (% fEPSP) was induced by high frequency stimulation (HFS) (100 Hz, 1 s). The average % fEPSP of the last 10 min was represented by bar graphs. Note that in PAE but not in control animals, Fe-NTA produced a significant decrease of LTP. (B) Effect of DFP on CA1 LTP of PAE and control rats. Slices were preincubated for 40 min with DFP and were continually perfused during the experiment. LTP experiment similar to A but in the presence of 100 μM of DFP. LTP (% fEPSP) was induced by high frequency stimulation (HFS) (100 Hz, 1 s). The average % fEPSP of the last 10 min was represented by bar graphs. Note that DFP significantly affected the LTP of control rats but not PAE rats. In all panels, sample traces were taken at the times indicated by numbers on the summary plot. The number of litter is indicated in parentheses. # Two way ANOVA C vs C DFP in temporal curse graph (B).**p value < 0.01, *p value < 0.05, repeated measured two way ANOVA followed Tukey’s post-hoc test. #p < 0.01 unpaired t test.