Table 1.
Nucleotide sequences of the forward (F) and reverse (R) primers used for qRT-PCR of genes involved in iron homeostasis and housekeeping genes in Rattus norvegicus.
| Gene | Sequences (5’-3’) | Amplicon (bp) | Reference |
|---|---|---|---|
| ratDMT1 (+) IRE | F: GCCTGTCTGTCTGTCTTTGC | 134 | (Pelizzoni et al., 2012) |
| R: CCCAGTGTTTCCCAACTAACA | |||
| ratDMT1 (-) IRE | F: AAGGCGAAGAAAGATCTGGAG | 113 | (Andersen et al., 2007) |
| R: CCACAGGCCGCTGTTTG | |||
| ratTFR1 | F: ATACGTTCCCCGTTGTTGAGG | 111 | (Naz et al., 2012) |
| R: GGCGGAAACTGAGTATGGTTGA | |||
| ratFPN1 | F: TCGGTTCCTCTCACTCCTGT | 198 | (Urrutia et al., 2013) |
| R: GTGGAGAGAGAGTGGCCAAG | |||
| ratHAMP | F: GAAGGCAAGATGGCACTAAGCA | 102 | (Moriconi et al., 2009) |
| R: TCTCGTCTGTTGCCGGAGATAG | |||
| ratBeta-actin | R: TCTACAATGAGCTGCGTGTG | 130 | (Haeger et al., 2010) |
| R: TACATGGCTGGGGTGTTGAA | |||
| ratGAPDH | F: AACGACCCCTTCATTGAC | 191 | (Dong et al., 2010) |
| R: TCCACGACATACTCAGCAC |