Fig. 4. Suitability of Acyl-cLIP for HTS campaigns. (A) Stopped signal from full 384-well plate of positive (HHAT) and negative (buffer) controls indicating excellent HTS suitability (Z′ = 0.69). (B) Screening of 775 FDA-approved drugs for inhibition of HHat, including RUSKI-201 as blind positive control. Hits defined as giving >80% inhibition at 25 μM.
