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. 2019 Nov 7;10:2550. doi: 10.3389/fimmu.2019.02550

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Copyright © 2019 Rodriguez-Cruz, Vesin, Ramon-Luing, Zuñiga, Quesniaux, Ryffel, Lascurain, Garcia and Chávez-Galán.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD3⁺TCRαβ⁺ MDM express chemokine receptors and tmTNF. Human monocyte-derived-macrophage were obtained at the 7th day post-culture and prepared for flow cytometry; the CD3⁺ MDM subpopulations were delimited. (A) Representative histogram showing the expression of CCR2 and CCR4 inside of each CD3⁺ MDM subpopulation. (B) Frequency of MDM subpopulations expressing chemokine receptors. (C) Representative histogram showing the expression of CD16 and tmTNF inside of each CD3⁺ MDM subpopulation. (D) Frequency of MDM subpopulation expressing CD16 and tmTNF. The bar graphs show the mean ± SD of n = 8–10 independent donors. A statistical analysis was performed by two-way ANOVA with multiple comparisons, following by a Tukey test. ****p < 0.0001.