Skip to main content
. 2019 Nov 7;10:2550. doi: 10.3389/fimmu.2019.02550

Figure 7.

Figure 7

CD3+TCRαβ+ MDM secrete IL-1β, IP-10, and MCP-1 by CD3- and TNF-dependent pathways. The supernatant of MDM subpopulations was recovered after 24 h in culture from unstimulated or stimulated by anti-CD3 or anti-TNF antibodies or anti-CD3 plus anti-TNF, isotype control and LPS plus IFN-γ, to perform a multiplex analysis. Pro-inflammatory cytokines IL-1β and IL-6 (A,B, respectively) and pro-inflammatory chemokines IP-10 and MCP-1 (C,D, respectively) were evaluated using Luminex technology. The data are expressed as the mean ± SD of n = 4–6 independent donors per condition. A statistical analysis was performed by multiple Bonferroni-Dunn comparisons. *p < 0.05, #p < 0.05, **p < 0.01, ##p < 0.01 and ####p < 0.0001 (*Indicates difference between groups, # indicates the difference compared with its negative control condition).