Figure 4.

Light microscopy images of ASCs forming tubular networks within PEGylated PFP hydrogels prepared using 5–12.5 U of thrombin (A) and 15–27 mM of CaCl₂ (B). The networks that formed from the ASCs increased in both thrombin and CaCl₂ hydrogels up to day 7. In comparison to CaCl₂ hydrogels, the networks formed within thrombin-based hydrogels appeared to be more robust at days 7 and 11. Scale bar = 100 μm. ASCs, adipose-derived stem cells. Color images are available online.