Figure 9.

Histological images of day 8 wound biopsies stained with MTS and polarized images of Picrosirius Red. (A) The low magnification ( × 40) of wounds treated with 23 mM CaCl₂- and 12.5 U thrombin-based PEGylated PFP hydrogels (±ASCs) stained with MTS. (B) The scanned images ( × 40) of the overall organization of collagen fibers in the granulating wound bed. The dashed white line on (B) was traced by superimposing bright field images onto the corresponding polarized image and indicates the areas of wound bed with any remnant hydrogels. The enlarged image to the right is designated by the white box. Scale bar = 40 μm. MTS, Masson's Trichrome stain. Color images are available online.