Skip to main content
. Author manuscript; available in PMC: 2019 Nov 14.
Published in final edited form as: J Cell Physiol. 2018 Aug 25;234(3):3105–3118. doi: 10.1002/jcp.27130

FIGURE 5.

FIGURE 5

Distribution of PP1γ2 testis enriched binding partners in soluble and insoluble fraction of sperm lysate. Western blot analysis was done to determine the supernatant versus pellet distribution of PPP1R2, PPP1R11, and PPP1R7 in sperm was done to determine the relative distribution of PPP1R2, PPP1R11, and PPP1R7, probed with the respective antibodies. (a) After sonication, PPP1R2 comes out mostly in the supernatant and is heat stable although there is some present in the pellet. (b,c) The proteins PPP1R11 and PPP1R7 are present in both sperm supernatant and pellet, although in higher amounts in the first