Skip to main content
. Author manuscript; available in PMC: 2019 Nov 14.
Published in final edited form as: J Cell Physiol. 2018 Aug 25;234(3):3105–3118. doi: 10.1002/jcp.27130

FIGURE 8.

FIGURE 8

Differential binding of PP1γ2 to PPP1R2, PPP1R7, and PPP1R11 in bull caput and caudal epididymal sperm. Immunoprecipitation s were done using PP1γ2, PPP1R7, PPP1R11, or PPP1R2 antibodies in bull caput and caudal sperm extracts and western blots were then probed with PPP1R7 or PP1γ2 antibodies. PP1γ2 antibody precipitates PPP1R2 and PPP1R7 from extracts of caudal but not from caput epididymal sperm. This is confirmed by reciprocal immunoprecipitation with a PPP1R7 antibody. Antibodies to PPP1R11 precipitate PP1γ2 from both caput and caudal sperm extracts. (Note: Western blots of PP1γ2 IP developed with PPP1R11 antibodies are not shown because the band for PPP1R11 runs close to the IgG light chain.)