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. 2019 Nov 13;52(6):622–630. doi: 10.1097/SHK.0000000000001319

Fig. 3.

Effects of N-acetylcysteine on oxidative damage in the kidneys of gentamicin-induced AKI mini pigs.

Fig. 3

(A) The levels of γ-H2AX, 4-HNE, and protein carbonyls in the kidney tissues of gentamicin-treated pigs were measured using western blotting. (B and C) Quantitative analysis of γ-H2AX and 4-HNE expression presented as mean ± SD (n = 6). ∗P < 0.05 vs. CON, #P < 0.05 vs. GEN. (D) Immunohistochemical staining for 8-OHdG in the kidneys of gentamicin-treated pigs (magnification, 400×). 4-HNE, 4-hydroxy-2-nonenal; 8-OHdG, 8-hydroxy-2′-deoxyguanosine; AKI, Acute kidney injury; CON, control group; GEN, gentamicin group; GEN + NAC, gentamicin + N-acetylcysteine group; NAC, N-acetylcysteine group; poly UB, polyubiquitinated protein aggregates.