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. 2019 Nov 13;52(6):622–630. doi: 10.1097/SHK.0000000000001319

Fig. 5.

Effects of N-acetylcysteine on apoptosis and the inflammatory response in gentamicin-injected AKI mini pigs.

Fig. 5

(A) The levels of cleaved caspase 3 were analyzed in kidney extracts from gentamicin-treated pigs using western blotting. (B) Quantitation of cleaved caspase-3 expression presented as mean ± SDs (n = 6). ∗P < 0.05 vs. CON, #P < 0.05 vs. GEN. (C) The number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) (+) cells was scored as described in the Methods section. ∗P < 0.05 vs. CON, #P < 0.05 vs. GEN. (D) TUNEL staining in kidney cortical sections (400×). (E–J) The mRNA expression levels of MCP-1, CCL-5, VCAM-1, CXCL2, IL-6, and ICAM-1 in mini pig kidneys were measured by quantitative PCR. Data are presented as mean ± SD (n = 6). ∗P < 0.05 vs. CON, #P < 0.05 vs. GEN. AKI, Acute kidney injury; CON, control group; GEN, gentamicin group; GEN + NAC, gentamicin + N-acetylcysteine group; NAC, N-acetylcysteine group; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling.