Fig 1. Fluorescent staining of early stages of Litoria caerulea PAK infection by Bd.

(A, E) Host cells and (B, F) Bd spores were visualised using a green and red cell tracker, respectively. (C, G) Nuclear content was stained with Hoechst and all pictures were merged in (D, H). (A-D) After 4 hours, initial contact was observed between host cells and Bd spores, as indicated by a white arrow (D). (E-H) 24 hours after inoculation, marked intracellular colonization was seen in Litoria caerulea host cells, as indicated by a white asterisk (H). Scale bar = 20 μm.