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. 2019 Oct 18;12:8653–8662. doi: 10.2147/OTT.S212352

Figure 4.

Figure 4

SNHG16 promotes the migration and invasion of retinoblastoma by sponging miR-182-5p and miR-128-3p to regulate LASP1.

Notes: (A) LASP1 level in retinoblastoma specimens and normal retina tissues determined using qRT-PCR and Immunohistochemical staining (magnification ×200, scale bar: 50 µm). (B) LASP1 level in retinoblastoma cell lines and human retinal pigment epithelial cell line determined using qRT-PCR. (C) and (D) Transwell assay results demonstrated that knockdown of LASP1 suppressed cell migration and invasion in RB50 and Y79 cells. (E) The predicted binding sites of LASP1 for miR-182-5p and miR-128-3p using TargetScan. (F) Luciferase activity of 293T cells co-transfected with either wild-type or mutant LASP1 luciferase vectors with miR-182-5p and miR-128-3p mimics or miRNA negative. (G) RB50 and Y79 cells were co-transfected si-SNHG16, si-NC with miR-182-5p inhibitor, miR-128-3p inhibitor or miRNA negative. LASP1 level was determined using Western blot. Data are expressed as mean ± SD of three independent experiments. **p<0.01 compared with the control group.