Figure 3.

Overshoot stimuli can account for fast-current decays observed by Corns et al. (2014). A, To mimic the displacement responses shown by Corns et al. (2014) (black traces; reproduced with permission from Corns et al. (2014)), we modified our step-like force stimulus (resulting displacement in magenta) to achieve an early plateau (arrow in onset zoom) in the displacement response using an overshoot stimulus (resulting displacement in blue). An expansion of the onset is shown to the right with the early plateau (arrow) and the oscillations (arrowheads) in the black trace indicated. B, The stimulus waveform (M), resulting hair bundle displacements, and the recorded currents are plotted. Black and Red traces highlight a positive and negative hair bundle displacement and resultant current, respectively. Using 1 mm intracellular BAPTA buffer, overshoot stimuli resulted in current decays that appear like fast adaptation (arrowheads). In the same cell, the fast-current decays were absent at positive potentials with overshoot stimuli. C, When removing the overshoot and using a step-like force stimulation in the same cell, no fast-current decays are evident at negative potentials. No averaging of stimulations was used, and current traces were 5 point smoothed using MATLAB's smooth function; displacement traces of 14 lines around the apex of the hair bundle were averaged.