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. 2019 Nov 13;39(46):9119–9129. doi: 10.1523/JNEUROSCI.0990-19.2019

Figure 2.

Figure 2.

Analysis of Rho family GTPases in GCPs. Activation of the Rho GTPases was analyzed in cultured GCPs treated with Shh ± proNT-3 for 48 h. A, Coimmunoprecipitation showing increased interaction between p75NTR and Rho-GDI with Shh treatment, which was decreased with proNT3 addition. Graph represents level of p75NTR pulled down by Rho-GDI immunoprecipitation relative to total Rho-GDI. *p = 0.0020 (one-way ANOVA, F(3,8) = 12.93). B, RhoA activation was increased by Shh and restored to basal levels with Shh + proNT3. **p = 0.0047 (one-way ANOVA with Tukey's post hoc test, F(2,9) = 10.28). C, Levels of active Cdc42 were not affected by any of the treatments. p = 0.9089 (one-way ANOVA followed by Tukey's post hoc test, F(2,6) = 0.09709). D, Active Rac1 in GCPs were unchanged in the presence of Shh or Shh + proNT3. p = 0.3004 (Tukey's post hoc test following a one-way ANOVA test, F(2,6) = 1.479). Error bars indicate mean ± SEM.