Conditioning-induced suppression to CS+ in the α′3 compartment persists <30 min. A, Change of odor response in α′3 axon segments between Post and Pre odor stimuli at 3, 15, 30, and 45 min after paired or unpaired training with MCH as CS+ and OCT as CS−. The change of odor response within each fly was calculated as (Post-Pre)/Pre. The paired training-induced suppression of the CS+ persisted for at least 15 min and became nonsignificant at 30 and 45 min. No significant difference was observed in CS− between paired and unpaired groups at any time point. Data are shown as mean ± SEM. ***p = 0.0002, **p = 0.0047; ns, not significant, p ≥ 0.2345; Mann–Whitney U test, n = 8. B, Change of odor response in the α′3 compartment between Post and Pre at 3, 15, 30, and 45 min after paired or unpaired training with OCT as CS+ and MCH as CS−. The paired training-induced suppression of the CS+ persisted for at least 15 min and became nonsignificant at 30 and 45 min. No significant difference was observed in CS− between paired and unpaired groups at any time point. Data are shown as mean ± SEM. ***p = 0.0006, *p = 0.0379; ns, not significant, p ≥ 0.1304; Mann–Whitney U test, n = 8.