Figure 6.

Suppression in α′3 is not induced by GABAergic inhibition through Rdl GABA_A receptor. A, No change in 3 min memory with 12 pulses of 90 V electric shock when Rdl (GABA_A receptor) was knocked down in α′β′ MBn. Data are shown as mean ± SEM. ns, Not significant; one-way ANOVA with Tukey's post hoc tests, n = 6. B, There was significant enhancement of 3 min memory expression with 12 pulses of 30 V electric shock when Rdl was knocked down in α′β′ MBn. Data are shown as mean ± SEM. *p ≤ 0.0391; one-way ANOVA with Tukey's post hoc tests, n = 6. C, Knocking down Rdl did not impair the odor suppression to the CS⁺ in the α′3 compartment. Left top, Time course of GCaMP6f response in the α′3 compartment to the CS⁺ (MCH) during a 5 s odor presentation before (Pre, blue) and 3 min after (Post, red) paired or unpaired training. Traces show the average response (±SEM) across all flies tested. Bottom, Mean odor-evoked Pre and Post responses during 5 s odor presentation. Right, Responses to the CS⁻ (OCT). Data are shown as mean ± SEM. ***p = 0.0009; ns, not significant, p ≥ 0.1515; repeated-measures two-way ANOVA with Bonferroni post hoc tests, n = 8. D, Control for Rdl knockdown in α′β′ MBn. Left top, Time course of GCaMP6f response in the α′3 compartment to the CS⁺ (MCH) during 5 s odor presentation before (Pre, blue) and 3 min after (Post, red) paired or unpaired training. Traces show the average response (±SEM) across all flies tested. Bottom, Mean odor-evoked Pre and Post responses during 5 s odor presentation. Right, Responses to the CS⁻ (OCT). Data are shown as mean ± SEM. ***p = 0.0006; ns, not significant, p ≥ 0.5049; repeated-measures two-way ANOVA with Bonferroni post hoc tests, n = 6.