Figure 10.

p53 regulates axon growth of developing cortical and hippocampal neurons. A, Representative images showing that inhibition of p53 activity with the pharmacological inhibitor, PFTα, blocked cortical neuron axon growth. Scale bar, 200 μm. B, Representative images showing that activation of p53 activity with the small molecule, tenovin-6, promoted cortical neuronal axon growth. Scale bar, 200 μm. C, Representative images showing that inhibition of p53 activity with the pharmacological inhibitor, PFTα, blocked hippocampal neuron axon growth. Scale bar, 200 μm. D, Representative images showing that activation of p53 activity with the small molecule tenovin-6, promoted hippocampal neuron axon growth. Scale bar, 200 μm. E, Quantification of the average axon lengths of cortical neurons shown in A from three independent experiments. p = 0.0006 for 5 μm vs 0 μm, p = 0.0002 for 10 μm vs 0 μm. F, Quantification of the average axon lengths of cortical neurons shown in B from three independent experiments. p = 0.0002 for 0.1 μm vs 0 μm, p = 0.0001 for 0.25 μm vs 0 μm. G, Quantification of the average axon lengths of hippocampal (Hippo) neurons shown in C from three independent experiments. p = 0.0261 for 5 μm vs 0 μm, p = 0.0007 for 10 μm vs 0 μm. H, Quantification of the average axon lengths of hippocampal neurons shown in D from three independent experiments. p = 0.0006 for 0.1 μm vs 0 μm, p = 0.0005 for 0.25 μm vs 0 μm. Data are represented as mean ± SEM. *p < 0.05, ***p < 0.001.