Figure 5.

Basal prelimbic mPFC calcium activity is unaffected by acute toluene. Activity was measured in prelimbic mPFC neurons with fiber photometry in rats injected with AAV1-CaMKII-GCaMP6f and exposed to air or toluene A, Exemplar viral expression (left) and fiber optic ferrule placement (right, black bars). B, Representative 15 min photometry recording following air (black) or toluene (red) treatment with detected calcium peak (blue arrows). Paired comparison of (C) GCaMP6f event frequency, (D) mean amplitude, (E) mean width, and (F) mean inter-event interval in toluene and control conditions in seven rats.