Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | MCFO-1 (pBPhsFLP2::PEST;+; UAS-FSF-smGdP::HA_V5_FLAG) | PMID: 25964354 | RRID: BDSC_64085 | Gift from Aljoscha Nern and Gerald Rubin |
| Genetic reagent (D. melanogaster) | 10XUAS-IVS-myr::tdTomato | Bloomington Drosophila Stock Center | RRID: BDSC_32222 | |
| Genetic reagent (D. melanogaster) | 23G12-GAL4 (T4/T5) | Bloomington Drosophila Stock Center | RRID: BDSC_49044 | T4/T5 driver |
| Genetic reagent (D. melanogaster) | 42F06-GAL4 (T4/T5) | Bloomington Drosophila Stock Center | RRID: BDSC_41253 | T4/T5 driver |
| Genetic reagent (D. melanogaster) | 23G12-LexA (T4/T5) | Bloomington Drosophila Stock Center | RRID: BDSC_65044 | T4/T5 driver |
| Genetic reagent (D. melanogaster) | {R59E08-p65ADZp (attP40); R42F06-ZpGdbd (attP2)} (T4/T5 splitGAL4) | PMID: 28384470 | JRC_SS00324 | |
| Genetic reagent (D. melanogaster) | UAS-CD4-tdGFP | Bloomington Drosophila Stock Center | RRID: BDSC_35839 | |
| Genetic reagent (D. melanogaster) | UAS-H2A::GFP | PMID: 26687360 | N/A | Gift from Barret Pfeiffer and Gerald Rubin |
| Genetic reagent (D. melanogaster) | LexAop-myr::tdTomato | PMID: 24462095 | N/A | |
| Genetic reagent (D. melanogaster) | 10XUAS-IVS-myr::GFP | Bloomington Drosophila Stock Center | RRID: BDSC_32197 | |
| Genetic reagent (D. melanogaster) | 10XUAS-IVS-mCD8::RFP | Bloomington Drosophila Stock Center | RRID: BDSC_32219 | |
| Genetic reagent (D. melanogaster) | Mi{PT-GFSTF.1}klg[MI02135-GFSTF.1] | Bloomington Drosophila Stock Center | RRID: BDSC_59787 | |
| Genetic reagent (D. melanogaster) | Mi{PT-GFSTF.1}beat-IV[MI05715-GFSTF.1] | Bloomington Drosophila Stock Center | RRID: BDSC_66506 | |
| Genetic reagent (D. melanogaster) | dpr2-Gal4 | Hugo J. Bellen | N/A | Gift from Hugo J. Bellen |
| Genetic reagent (D. melanogaster) | P{w[+mW.hs]=GawB}grn[05930-GAL4] | Bloomington Drosophila Stock Center | RRID: BDSC_42224 | |
| Genetic reagent (D. melanogaster) | Mi{y[+mDint2]=MIC}beat-VI[MI13252] | Bloomington Drosophila Stock Center | RRID: BDSC_58680 | |
| Genetic reagent (D. melanogaster) | P{y[+t7.7] v[+t1.8]=TRiP.HMS01085}attP2 | Bloomington Drosophila Stock Center | RRID: BDSC_33746 | UAS-grnRNAi |
| Genetic reagent (D. melanogaster) | P{UAS-p35.H}BH1 | Bloomington Drosophila Stock Center | RRID: BDSC_5072 | |
| Genetic reagent (D. melanogaster) | UAS-grn.ORF.3xHA | FlyORF | Stock #: F001916 | |
| Antibody | Chicken polyclonal anti-GFP | Abcam | Cat. #: ab13970 RRID: AB_300798 |
IHC (1:1000) |
| Antibody | Rabbit polyclonal anti-dsRed | Clontech | Cat. #: 632496 RRID: AB_10013483 |
IHC (1:200) |
| Antibody | Mouse monoclonal anti-Brp | Developmental Studies Hybridoma Bank | Cat. #: nc82 RRID: AB_2314866 |
IHC (1:20) |
| Antibody | Mouse monoclonal anti-V5 | Abcam | Cat. #: ab27671 RRID: AB_471093 |
IHC (1:300) |
| Antibody | Rabbit polyclonal anti-Dcp-1 | Cell Signalling | Cat. #: 9578 RRID: AB_2721060 |
IHC (1:50) |
| Antibody | Goat polyclonal anti-chicken IgY Alexa Fluor 488 | Invitrogen | Cat. #: A11039 RRID: AB_142924 |
IHC (1:200) |
| Antibody | Goat polyclonal anti-mouse IgG Alexa Fluor 488 | Invitrogen | Cat. #: A11029 RRID: AB_138404 |
IHC (1:500) |
| Antibody | Goat monoclonal anti-rabbit IgG Alexa Fluor 568 | Invitrogen | Cat. #: A11011 RRID: AB_143157 |
IHC (1:200) |
| Antibody | Goat polyclonal anti-rat IgG Alexa Fluor 568 |
Invitrogen | Cat. #: A11077 RRID: AB_141874 |
IHC (1:500) |
| Antibody | Goat oligoclonal anti-rabbit IgG Alexa Fluor 647 | Invitrogen | Cat. #: A27040 RRID: AB_2536101 |
IHC (1:200) |
| Antibody | Donkey polyclonal anti-mouse IgG Cy5 | Jackson ImmunoResearch Laboratories | Cat. #: 715-175-150 RRID: AB_2340819 |
IHC (1:200) |
| Chemical compound, drug | Papain | Worthington | Cat. #: LK003178 | |
| Chemical compound, drug | Liberase protease | Sigma-Aldrich | Cat. #: 5401119001 | |
| Software, algorithm | Cell Ranger 2.2.0 | https://10xgenomics.com | RRID:SCR_017344 | |
| Software, algorithm | Seurat 2.3.4 | https://satijalab.org/seurat/ | RRID: SCR_016341 |