Figure 1. ssDNA pipeline is faster than cloning CRIMIC constructs.

(A) Schematic of PCR-based generation of drop-in ssDNA constructs. Gray boxes, UTRs; orange boxes, coding exons; yellow line, coding introns; black line, outside coding introns and exons. (B) Comparison of donor generation pipelines for CRIMIC and PCR-based drop-in ssDNA homology donors. Making ssDNA donors is about 5X faster than making CRIMIC donors.