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. 2019 Nov 1;8:e51539. doi: 10.7554/eLife.51539

Figure 6. Single step cloning method allows efficient insertion of the CRIMIC cassette in coding introns.

(A) Schematic of a single step cloning vector pUC57. LHA Left Homology Arm, RE1 Restriction Enzyme 1, RE2 restriction Enzyme 2, RHA Right Homology Arm. (B) Injection results for the seven genes selected to estimate transformation efficacy. Numbers indicate positive events/G0 single fly crosses. (C) Third instar larval brain expression domain of CG5009 as determined by crossing conventional CRIMIC or drop-in int100-CRIMIC flies to UAS-NLS-mCherry reporter lines. Scale bar is 100 µm.

Figure 6.

Figure 6—figure supplement 1. Comparison of expression domain obtained by T2A-miniGAL4 and drop-in int100-CRIMIC.

Figure 6—figure supplement 1.

Third instar larval brain expression domain of Khc as determined by crossing conventional T2A-miniGAL4 or drop-in int100-CRIMIC flies to UAS-NLS-mCherry reporter lines. Scale bars are 100 µm.