Table 1. Summary of ssDNA drop-in mediated homologous recombination in S2R+ cells.
| Organelle | Fly protein | clones obtained | population imaged | clones imaged | insertion sequence verified | Immunostained | Correct GFP localization | DGRC stock# | S2R+ expression (modENCODE RPKM) |
|---|---|---|---|---|---|---|---|---|---|
| Autophagosomes | Atg8a | 14 | ✔ | ✔ | N | N | N | N | 153 |
| Autophagosomes/aggregates | Ref(2)P | 9 | ✔ | ✔ | Y | a-Ref2P, a-FK2 | N | N | 138 |
| Endoplasmic reticulum (ER) | Calnexin99A | 16 | ✔ | ✔ | Y | a-Cnx99a | Y | 273 | 235 |
| Endoplasmic reticulum (ER), transitional | Sec23 | 30 | ✔ | ✔ | Y | N | * | 294 | 101 |
| Endosomes, early | Rab5 | 9 | ✔ | ✔ | N | N | N | N | 98 |
| Endosomes, recycling | Rab11 | 23 | ✔ | ✔ | Y | N | * | 274 | 302 |
| G-Bodies (cytoplasmic puncta) | Pfk | 14 | ✔ | ✔ | N | N | N | N | 23 |
| Golgi (cis-Golgi) | Gmap | 10 | ✔ | ✔ | Y | a-GMAP | Y | 276, 277 | 15 |
| Golgi (trans-Golgi) | Sec71 | 10 | ✔ | ✔ | N | N | N | N | 8 |
| Golgi (trans-Golgi) | Golgin245 | 1 | ✔ | ✔ | Y | a-Golgin245 | Y | 280 | 27 |
| Kinetochore | Polo | 2 | ✔ | ✔ | Y | N | Y | 275 | 50 |
| Lipid droplets | Seipin | 12 | ✔ | ✔ | N | N | N | N | 9 |
| Lysosomes | spin | 2 | ✔ | ✔ | Y | a-Arl8 | Y | 293 | 112 |
| Lysosomes | Arl8 | 9 | ✔ | ✔ | Y | a-Arl8 | Y | 291 | 78 |
| Mitochondria | Tim17b | 3 | ✔ | ✔ | N | a-ATP5A | N | N | 266 |
| Mitochondria | Tom20 | 17 | ✔ | ✔ | Y | a-ATP5A | Y | 302 | 117 |
| Nuclear membrane, inner | dLBR | 0 | ✔ | N | N | N | N | N | 42 |
| Nuclear membrane, inner | Lamin | 53 | ✔ | ✔ | Y | a-Lamin | Y | 292 | 249 |
| Nucleolus | Fibrillarin | 14 | ✔ | ✔ | Y | a-Fib | Y | 278, 279 | 53 |
| Peroxisomes | Pmp70 | 6 | ✔ | ✔ | N | N | N | N | 26 |
* = distribution was as expected, but no antibody available to test by co-stain.