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. 2019 Nov 13;8:e46302. doi: 10.7554/eLife.46302

Figure 5. Effects of TetraUb on nucleotide exchange.

Figure 5.

Nucleotide exchange kinetics in the absence or presence of Lys63 linked tetra ubiquitin. Panel (A) shows an example data trace for WT Rabex5:Rabaptin5 complex alone (dark blue) and plus tetraUb (light blue). Panel (B) shows an example data trace for RabexΔUb:Rabaptin5 complex alone (dark rust) and plus tetraUb (light rust). Panels (C) and (D) show the average of 2 experiments each containing three replicates for WT Rabex5:Rabaptin5 complex (blue squares) and RabexΔUb:Rabaptin5 complex (rust diamonds) with varying concentrations of tetraUb.

Figure 5—source data 1. Nucleotide exchange kinetics +/- Ubiquitin.
elife-46302-fig5-data1.xlsx (128.6KB, xlsx)