Table 1. ELIC WT channel properties by patch-clamping giant liposomes composed of varying mole% POPG.
The rate and extent of desensitization are reported as weighted time constants (τ), and the current after 20 s of 30 mM cysteamine application normalized to peak response. Also shown are activation time constants (τ) in response to 30 mM cysteamine and EC50s for cysteamine activation (n = 3–5, ± SD).
| Desensitization | Activation | Dose response | ||
|---|---|---|---|---|
| POPG | Weighted τ (s) | Norm current at 20 s | τ (ms) | EC50 (mM cysteamine) |
| 12% | 0.93 ± 0.32 | 0.05 ± 0.02 | 112 ± 29 | 5.3 ± 1.0 |
| 25% | 1.95 ± 0.47 | 0.10 ± 0.04 | 134 ± 50 | 5.1 ± 1.2 |
| 40% | 2.98 ± 0.24 | 0.21 ± 0.03 | 133 ± 66 | 6.5 ± 1.3 |