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. 2019 Apr 16;111(11):1202–1215. doi: 10.1093/jnci/djz051

Figure 5.

Figure 5.

Comparison of estrogen receptor-beta (ESR2) effects in triple negative breast cancer cells expressing mutant vs wild-type (WT) TP53. A) TP53-target gene expression in MDA-MB-231 with or without knocking down ESR2 with siRNA #2 for 48 hours was determined by quantitative real-time PCR (qRT-PCR). B) TP53-target gene expression in MDA-MB-23-TP53KO with or without knocking down ESR2 with siRNA #2 for 48 hours was determined by qRT-PCR. C) TP53 target gene expression in MDA-MB-231-TP53KO cells following ESR2 knockdown along with or without transfection with WT TP53 cDNA for 48 hours was determined by qRT-PCR. D) TP53-target gene expression in BC3-WT TP53 cells with or without knocking down ESR2 with ESR2 siRNA #2 for 48 hours was determined by qRT-PCR. Right panel: Expression of CDKN1A, BBC3, and BID proteins in BC3-WT TP53 cells transfected with or without knocking down ESR2 with siRNA #2 for 48 hours was analyzed by immunoblotting. E) TP53-target gene expression in BC3-WT TP53 cells 48 hours post-transfection with or without FLAG-ESR2 was determined by qRT-PCR. F) TP53-target gene expression in BC3-shTP53 cells with and without knocking down ESR2 with siRNA #2 for 48 hours was analyzed by qRT-PCR. G) TP53-target gene expression in CAL-51 cells 48 hours post-transfection with or without FLAG-ESR2 was determined by qRT-PCR. Right panel: Expression of CDKN1A1 and BBC3 proteins in BC3-WT TP53 cells transfected with or without knocking down ESR2 with ESR2 siRNA #2 for 48 hours was analyzed by immunoblotting. All P values were determined by two-tailed Student t test. Error bars represent SD. si-NS = non-specific siRNA; siESR2 = ESR2-specific siRNA; Cl.BID = cleaved BID; Cl.BCL2L11 = cleaved BCL2L11.