Figure 7.

Sensitivity of Tegavivint in metastatic lung patient-derived xenograft-derived ALDH1^high cells with respect to viability and self-renewal properties in vitro. A) TCCC-OS84 cells were sorted into ALDH1^high and ALDH1^low populations. N, N-diethylaminobenzaldehyde (DEAB) is an ALDH1 inhibitor used as a negative control. SSC = side scatter. FITC=Fluorescein isothiocyanate B) Quantitative polymerase chain reaction (qPCR) analysis for expression of ALDH1A1, β-catenin, TCF4, CD44, c-Myc, Oct3/4, Sox2, and Nanog in TCCC-OS84 ALDH1^high and ALDH1^low cell populations (n = 6). Data are shown as median with 95% confidence interval. Two-sided Student t test was used to determine statistical significance. C) Effect of Tegavivint treatment for 24 hours on cell viability of ALDH1^high and ALDH1^low cells (n = 8) is shown. Data are shown as median with 95% confidence interval. Two-sided Student t test was used to determine statistical significance. D) Representative images of primary sarcospheres 6 days after seeding ALDH1^low or ALDH1^high cells are shown. Scale bar = 100 µm. E) Low- and high-magnification images of secondary sarcospheres 6 days after seeding ALDH1^high cells with or without pretreatment with 100 nM Tegavivint are shown. The average number of sarcospheres in each group (n = 6/group) was quantified. Data are shown as median with 95% confidence interval. Two-sided Student t test was used to determine statistical significance. Scale bar = 100 µm.